Abstract: 9.30 In Vitro Observation Of 15 Cm Long Artificial Nerve Grafts Seeded With Schwann Cells
نویسندگان
چکیده
IntroductIon: Peripheral nerve injury is a common and debilitating consequence of traumatic injury. Surgical repair of peripheral nerve by repositioning results in a degree of nerve regeneration and functional recovery. However, the clinical outcome, particularly in long length nerve defects, is disappointing and research continues to develop approaches to optimize functional recovery. An approach we are investigating is the use of fine spider silk fibers from species Nephilla Edulis for guidance material for peripheral nerve regeneration. Aim of this small study was to investigate cell survival, cell migration and distribution of nutritive substances on spider silk through an artificial nerve graft of various lengths ranging from 04-15cm.
منابع مشابه
Electrophysiological Study of Sciatic Nerve Regeneration Through Tubes Seeded with Schwann Cells
A B S T R A C TIntroduction: Peripheral nerve injury is a common disorder and leads to permanent neurological defects. Schwann cells have been shown to have nerve repair after being transplanted in peripheral nerve injury. The aim of this study was to determine the beneficial effect of allograft Schwann cells on electrophysiological outcome after transection of the sciatic nerve in rats.Methods...
متن کاملCharacterization and Schwann Cell Seeding of up to 15.0 cm Long Spider Silk Nerve Conduits for Reconstruction of Peripheral Nerve Defects
Nerve reconstruction of extended nerve defect injuries still remains challenging with respect to therapeutic options. The gold standard in nerve surgery is the autologous nerve graft. Due to the limitation of adequate donor nerves, surgical alternatives are needed. Nerve grafts made out of either natural or artificial materials represent this alternative. Several biomaterials are being explored...
متن کاملRecovery of erectile function comparing autologous nerve grafts, unseeded conduits, Schwann-cell-seeded guidance tubes and GDNF-overexpressing Schwann cell grafts
Dissection of the cavernous nerves during radical prostatectomy for prostate cancer eliminates spontaneous erections. Using the rat as an experimental model, we compared the regenerative capacity of autologous nerve grafts and Schwann-cell-seeded nerve guides. After bilateral excision of cavernous nerve segments, cavernous nerves were reconstructed using unseeded silicon tubes, nerve autografts...
متن کاملEfficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures
Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The tot...
متن کاملEvaluation of the Functional Recovery in Sciatic Nerve Injury following the Co-transplantation of Schwann and Bone Marrow Stromal Stem Cells in Rat
Introduction: Transplantation of bone marrow stromal cells (BMSCs) or Schwann cells (SCs) can increase axonal regeneration in peripheral nerve injuries. Based on our previous investigations, the goal of the present work was to examine the individual and synergistic effects of the two different cell types in sciatic nerve injury . We pursued to evaluate the effects of BMSCs and SCs co-transplant...
متن کامل